other:Article Title: Chronic, cortex-wide imaging of specific cell populations during behavior
Article Snippet: Alternatively, the macroscope height can be adjusted with a focusing module (Thorlabs, cat. no. ZFM1020) Macroscope excitation light and wavelength alternation ● GCaMP excitation LED, 470 nm (Thorlabs, cat. no. M470L4) ● Hemodynamic correction LED, 405 nm (Thorlabs, cat. no. M405L4) ● 2× Adjustable collimation adapter (Thorlabs, cat. no. SM2F32-A) ● 2× T-cube LED driver (Thorlabs, cat. no. LEDD1B) ● Teensy 3.2 (PJRC, cat. no. TEENSY32) ● SMA female RF coaxial adapter PCB mount plug (Amazon, ASIN B06Y5WZ1TK) ● USB to micro USB, 6 ft (Digikey, cat. No. AE10342-ND) ● Prototyping perforated breadboard 2′′ × 3.2′′ (Adafruit, cat. no. 1609) ● BNC connector jack, female socket 75 Ohm panel mount (Digikey, cat. no. A97562-ND) ● Adapter coaxial connector, female socket to BNC jack (Digikey, cat. no. ARFX1069-ND) ● Coaxial BNC to BNC male to male RG-58 29.53′′, 750.00 mm (Digikey, cat. no. J10339-ND) ● Coaxial SMA to SMA male to male RG-316 5.906′′, 150.00 mm (Digikey, cat. no. J10284-ND) ● BNC, male plug to wire lead 72.0′′, 1,828.80 mm (Digikey, cat. no. 501-2556-ND)
Article Title: Chronic, cortex-wide imaging of specific cell populations during behavior
Article Snippet: Alternatively, the macroscope height can be adjusted with a focusing module (Thorlabs, cat. no. ZFM1020) Macroscope excitation light and wavelength alternation GCaMP excitation LED, 470 nm (Thorlabs, cat. no. M470L4) Hemodynamic correction LED, 405 nm (Thorlabs, cat. no. M405L4) 2× Adjustable collimation adapter (Thorlabs, cat. no. SM2F32-A) 2× T-cube LED driver (Thorlabs, cat. no. LEDD1B) Teensy 3.2 (PJRC, cat. no. TEENSY32) SMA female RF coaxial adapter PCB mount plug (Amazon, ASIN B06Y5WZ1TK) USB to micro USB, 6 ft (Digikey, cat. No. AE10342-ND) Prototyping perforated breadboard 2′′ × 3.2′′ (Adafruit, cat. no. 1609) BNC connector jack, female socket 75 Ohm panel mount (Digikey, cat. no. {"type":"entrez-protein","attrs":{"text":"A97562","term_id":"25318253","term_text":"pir||A97562"}} A97562 -ND) Adapter coaxial connector, female socket to BNC jack (Digikey, cat. no. ARFX1069-ND) Coaxial BNC to BNC male to male RG-58 29.53′′, 750.00 mm (Digikey, cat. no. J10339-ND) Coaxial SMA to SMA male to male RG-316 5.906′′, 150.00 mm (Digikey, cat. no. J10284-ND) BNC, male plug to wire lead 72.0′′, 1,828.80 mm (Digikey, cat. no. 501–2556-ND) Other Data acquisition device to synchronize with behavior setups (National Instruments, USB-6001, cat. no. 782604-01) Power meter (Thorlabs, cat. no. PM100D) Photodiode power sensor, 400–1,100 nm (Thorlabs, cat. no. S120C) 3D-printed light-shielding cone (custom piece printed from Shapeways ( https://www.shapeways.com ) in opaque plastic (e.g., Multi Jet Fusion PA 12, design files in https://github.com/churchlandlab/WidefieldImager/blob/master/CAD/LightCone/LargerWidefieldCone_v3.stl ) Data acquisition computer, at least 4-core CPU with 32 GB RAM, with a full-length PCI slot for the frame grabber and a separate solid state drive for data acquisition Software MATLAB 2016b or newer, image/data acquisition toolboxes ( https://www.mathworks.com/ ) PCO frame grabber drivers ( https://www.pco.de/fileadmin/user_upload/pco-driver/PCO_SISOINSTALL_5.7.0_0002.zip ) PCO Camware ( https://www.pco.de/fileadmin/user_upload/pco-software/SW_CAMWAREWIN64_410_0001.zip ) PCO SDK ( https://www.pco.de/fileadmin/user_upload/pco-software/SW_PCOSDKWIN_125.zip ) PCO MATLAB toolkit ( https://www.pco.de/fileadmin/user_upload/pco-software/SW_PCOMATLAB_V1.1.2.zip ) Recording software: https://github.com/churchlandlab/WidefieldImager ▲ CRITICAL A fully functioning Python alternative can be found here: https://bitbucket.org/jpcouto/labcams Preprocessing and graphical interface: https://github.com/churchlandlab/wfield NeuroCAAS computing platform: http://neurocaas.org Reagent setup !
Article Title: Chronic, cortex-wide imaging of specific cell populations during behavior
Article Snippet: Scalpel (Fine Science Tools, cat. no. 10003-12) Forceps (Fine Science Tools, cat. no. 11049-10) Scissors (Fine Science Tools, cat. no. 14040-10) Vannas scissors (World Precision Instrument, cat. no. 501777) Micro probe, angled (Fine Science Tools, cat. no. 10032-13) Delicate bone scraper (Fine Science Tools, cat. no. 10075-16) Fine forceps (Fine Science Tools, cat. nos.
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